Multichannel incubation type planar patch clamp biosensor using plastic (PMMA) substrates and characterization by laser-gated ion-channel protein
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چکیده
Recently we have developed 4 channel incubation type planar patch clamp biosensor with polymethylmethacrylate (PMMA) substrate by using both-side hot embossing, focused ion beam micro-fabrication and surface modification by extra cellular matrix. We have evaluated the performance by using the light-gated ion-channel protein, channelrhodopsin wide receiver (ChRWR) expressed on HEK293 cell. Observed laser-induced current profile and the membrane potential dependences well agreed to those observed by pipette patch clamp, suggesting its excellent possibility in important applications such as portable ion channel biosensors and high throughput screening with neural systems.
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تاریخ انتشار 2012